Rules for sampling of transported (transported) objects and biological material
The general procedure for samples of biological material for laboratory studies of food products is carried out in accordance with the Order of the Minister of Agriculture of the Republic of Kazakhstan dated April 30, 2015 No. 7-1/393 On Approval of the rules for sampling transported (Transported) objects and biological material.
These Rules for Sampling of Relocated (transported) objects and Biological Material (hereinafter referred to as the Rules) have been developed in accordance with subparagraph 34) of Article 8 of the Law of the Republic of Kazakhstan dated July 10, 2002 “On Veterinary Medicine” and determine the procedure for sampling of relocated (transported) objects and biological material.
Procedure for sampling of transported (transported) objects and biological material
The samples taken within a period of no more than 24 hours for perishable products and for others – 36 hours are sent to veterinary laboratories with a cover letter (moved (transported) objects) in the form according to Annex 4 to these Rules and an act of sampling of moved (transported) objects in the form according to Annex 5 to these Rules (hereinafter – Act). The act is drawn up in three copies: the first copy is intended to be sent together with the samples to the veterinary laboratory, the second remains with the state veterinary and sanitary inspector or the state veterinarian who conducted the sampling, the third copy of the sampling act is transferred to the owner of the moved (transported) object or his representative.
Sampling of biological material and its delivery to the veterinary laboratory are carried out by specialists in the field of veterinary medicine, with the preparation, depending on the type, of cover letters (biological or pathological material) according to the forms, according to Annexes 6 and 7 to these Rules and the act of sampling of biological material (with the exception of taking blood samples) according to the form, according to Annex 8 to these Rules with the appendix of the inventory of sampling of biological material in the form, according to Annex 9 to these Rules.
Samples are delivered to the veterinary laboratory in compliance with measures against leakage, drying, decomposition, mutual contamination of samples in compliance with the temperature regime. During the transportation of samples, precautions are taken to prevent contamination of samples with foreign odors, direct sunlight, and environmental safety. Samples of perishable samples are placed in a cooler bag (thermochem) or covered with dry ice. Each sample is provided with a label or tag (it remains until the end of the research), which indicates the type of sample, the mass of the sample, the date and place of sampling, the surname and initials of the person who conducted the sampling in the state and Russian languages. On test tubes or other systems for sampling biological material with samples by means of a pencil (marker) on the glass, numbering is affixed, according to the attached inventory of sampling biological material or by sticking a sticker with barcodes of the individual number of the animal.
The control sample is allocated on the spot during the sampling process in a sterile container and in a sealed (sealed) form should be stored in the veterinary laboratory that conducted the research. The shelf life of control samples is at least 14 days from the end of laboratory tests, and for samples that do not meet the established requirements, no more than three months, depending on the purpose of the study, obtaining the result of the conclusion according to the act of examination (test protocol). For perishable products, the shelf life of the control sample, for a number of quality and safety indicators (organoleptic and microbiological) cannot be longer than its shelf life.
Features of sampling of animal products
Sampling of meat of all types of slaughter animals (beef, lamb, pork and from other types of agricultural and commercial animals with the exception of rabbits, poultry, fish) is carried out in the following order.
Spot samples of meat (without fat) from the carcass or half–carcass are taken in sterile pieces after preliminary cauterization of the incision site of at least 200 grams in one of the following places – at the incision site, in the scapula area, in the thigh area from thick parts of the muscles.
To carry out organoleptic methods for determining the freshness of meat, a combined sample is formed, from which an average sample weighing at least 200 grams is isolated.
To determine the total radiation background of samples, dosimetric monitoring is carried out using dosimetric devices without sampling.
To carry out radiation monitoring by spectrometric devices, toxic elements, and the residual amount of antibiotics, an average sample weighing at least 1 kilogram is taken from the combined sample.
For microbiological indicators , a part of the flexor and extensor muscles of the anterior and posterior extremities is selected by the size of 8*6*6 , whole lymph nodes (superficial cervical and knee folds with surrounding tissue), tubular bone (if necessary) form a combined sample, allocate an average sample for the study of 250 grams.
To study for trichinosis, samples are taken from each carcass, two samples from the legs of the diaphragm, and in the absence of them, a combined sample is formed from the muscular costal part of the diaphragm, intercostal muscles or neck muscles, and an average sample of at least 60 grams is isolated from it.
Samples from frozen blocks of meat are taken in pieces weighing at least 200 grams. To check the quality, weight and temperature of frozen meat blocks, 10% of the packaging places are sampled, but not less than 10 places from each batch, the temperature is measured with liquid thermometers (not mercury) with a temperature scale from 0 to 1000 ° C in the thickness of the pectoral and femoral muscles.
At meat processing plants, slaughterhouses, refrigerators and temporary storage warehouses, the sample includes no more than 10% of carcasses (half carcasses) of cattle, 5% of carcasses of sheep, pigs and 2% of frozen or chilled blocks of meat from a homogeneous batch. Spot samples from frozen and chilled blocks of meat are also taken in whole pieces of at least 200 grams.
From the obtained point samples, an average sample from the batch is formed, guided by the norms for selecting the number of average samples of livestock products from the batch, according to Annex 3 to these Rules.
Meat sampling of chickens, ducks, rabbits is carried out by carcasses or half carcasses, geese and turkeys – quarters of carcasses. Bird carcasses are selected from the batch supplied for sale by random sampling. A combined sample is formed from point samples, average samples from the batch are isolated from the combined sample, guided by the sampling standards from the batch of poultry, rabbits according to Annex 1 to these Sampling Features of the transported (transported) object and biological material (hereinafter referred to as Sampling Features).
To carry out radiation monitoring with spectrometric devices, a combined sample is formed from point samples, an average sample of at least 1 kilogram is isolated from it. millimeters, dense honey – dipstick for oil from different layers, a point sample of 100 grams.
To conduct laboratory tests of honey for organoleptic indicators, an average sample of 100 grams, radiation control, an average sample weighing at least 1 kilogram from a batch of honey is isolated, a sample of packaging units is made, where honey sampling standards are guided according to Appendix 2 to these Sampling Features.
Before sampling, the milk in the containers is thoroughly mixed. To control the quality of milk and dairy products in tanks, point samples of 250 milliliters are taken according to organoleptic, physico-chemical and microbiological indicators to form a combined sample, the volume of the average sample is 1 liter.
To carry out radiation monitoring, the content of toxic elements, and the residual amount of antibiotics, an average sample weighing at least 2 liters is taken from the combined sample.
To control the quality of milk and dairy products in tanks, a combined sample is taken from each batch of the product according to physico-chemical and microbiological indicators. The volume of the combined sample is not more than 1.5 liters.
To determine the compliance of eggs with veterinary (veterinary and sanitary) requirements and safety requirements, a sample of packaging units is made from the batch. To compile a combined and separate an average sample from it, the standards for sampling eggs from packaging units in a batch are guided, according to Annex 4 to these Sampling Features.
When selecting a batch for the formation of a combined, then average sample, the standards for sampling eggs from the number of pieces are guided, according to Annex 5 to these Sampling Features.
Samples are taken from domestic eggs from different points in the amount of: up to 50 eggs – 4 pieces, up to 100 – 6 pieces, up to 1000 – 10 pieces, up to 2000 – 15 pieces, up to 3000 – 20 pieces.
Samples of fish and fish products are taken from different places that most characterize the batch, 10% of packages are included in the sample. To control a live, fresh chilled batch, 1-2% of fish by weight are selected. All types of fish are subject to research separately.
Spot samples from small specimens of fish are taken in whole carcasses in the amount of:
1) with the weight of one fish up to 100 grams – 5-7 pieces from each package;
2) with the weight of one fish up to 1 kilogram – 2 samples of 100 grams from 1-2 fish from each package;
3) if the weight of one fish is more than 1 kilogram – from 3 fish, separate pieces, each 5 centimeters wide from the head and back, form a combined sample, allocate an average sample of at least a kilogram, for expensive fish no more than 500 grams.