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International cooperation and joint projects

International cooperation and joint projects

Scientific projects and agreements of Republican State Enterprise (RSE) "NRTSV"

RSE "NRTSV" was directly active in various research projects in collaboration with scientific institutes of the Republic of Kazakhstan and other countries.

1.      Project ''Twinning lab'' is one of the international projects in the period of 2013-2015., Which was directly involved in RSE "NRTSV" as a candidate for the OIE international reference laboratory for brucellosis status in the Central Asian region on the basis of an agreement on the program of cooperation in the veterinary field between the Government of the Republic of Kazakhstan and the OIE. The agreement was signed during the period from 9 to 13 July 2012 during the official visit of the OIE Director General Dr. Bernard Vallat to the Republic of Kazakhstan.

The project strated operating actively since December 2013 with collaboration laboratory, which is the current international OIE reference laboratory for brucellosis and an applicant laboratory for the acquisition of status, currently operates under the name «Twinning lab».

The project was financed by the International Epizooties Bureau (OIE), Paris, France, which includes the supply of reagents and materials required for the purposes of the project and international professional training at the Zoological Institute in Teramo, Italy.

1.1 The purpose of the project is to provide RSE "NRTSV" the OIE status as an international reference laboratory for brucellosis in the Central Asian region. Zoological Institute (IZSAM) Teramo, Italy and Italian Ministry of Health together with a team of scientists with extensive international experience became collaborators of the project. IZSAM Institute cooperates with many international institutions in foreign countries (90 countries) and international organizations such as WHO, FAO and OIE. The main project areas are located in Western Europe, Latin America, Africa and Asia. The main objective of the IZSAM Institute is the prophylaxis of animal diseases to prevent human infection. 

1.2 Project Objectives to increase the level of technical expertise of RSE "NRTSV" personnel, the introduction of methods and standards recommended by the OIE in RSE "NRTSV", on the whole territory of the Republic of Kazakhstan; introduction of methods to create a collection of national reference materials (standards) with the use of generally accepted international validation procedures; to hold the events and workshops for all veterinary laboratories in Kazakhstan and neighboring countries.

Objectives to improve the level of technical knowledge of the personnel of RSE "NRTSV" include the following training activities of the laboratory:

- Serological diagnosis of brucellosis;

- Bacteriological procedures for the isolation and characterization of Brucella;

- Molecular apparatus to identify Brucella;

- The epidemiological status of animal brucellosis;

- Quality control of the results;

- Validation of test methods and test results;

- Biosafety procedures;

- Trainings and publications.

Events

Implementation period

Participants

1.

To obtain esults from the carried out in December 2014 collation tests for serological studies organized by IZSAM.

Analysis of the results.

January 2015

Specialists of the DIZ laboratory serology department

2.

Forward IZSAM the isolated Brucella DNA samples to compare the results obtained in NRTSV for further MLVA analysis.

February 2015

Specialists of the Department of Molecular Biology

3.

The workshop on laboratory studies of brucellosis, based on the knowledge gained.

February 24-26 2015

Specialists of 7 branches of the RGP "RVL" areas: East Kazakhstani region, South Kazakhstani region, Almaty, Kyzylorda, Karaganda, Zhambyl, Akmola. Specialists Department of brucellosis of RSE "KazNIVI"

3.1

Carrying serology seminar on "Serological diagnosis of brucellosis methods according to international standards".

3.2

Bacteriology workshop on the topic of "Implementation of bacteriological research method recommended by OIE brucellosis".

3.3

Molecular genetics workshop on the topic of "Implementation of molecular biological research methods for the diagnosis of brucellosis, Brucella typing and biotyping".

4.

Diagnostic work on brucellosis - collection of blood serum, pathological material on the territory of the Republic of Kazakhstan, isolation of pure culture, genotyping, collation test.

February-September 2015

DIZ Laboratory RSE "NRTSV"

5.

The workshop on laboratory studies of brucellosis, based on the knowledge gained in IZSAM for veterinary laboratories and specialists in Kazakhstan and neighboring states - Kyrgystan, Uzbekistan, Turkmenistan, Tajikistan

June 10-12, 2015.

Specialists of 7 branches RGP "RVL" from the areas of: Pavlodar, North Kazakhstan, Atyrau, Mangistau, Aktobe, Kostanay, West Kazakhstan. Specialists Department of brucellosis RSE "KazNIVI"

5.1

The workshop on "Serological diagnosis of brucellosis methods according to international standards"

5.2

Bacteriology seminar on the topic of "Implementation of bacteriological research method recommended by OIE brucellosis"

5.3

Molecular genetics seminar on the topic of "Implementation of molecular biological research methods for the diagnosis of brucellosis, Brucella typing and biotyping"

5.4

Epidemiology seminar on the topic of "Analysis of the epizootic status for brucellosis in the Republic of Kazakhstan for 2014"

6.

Implementation of the interim audit work with the demonstration of acquired knowledge, skills, results on the project "Twining Laboratory" IZSAM visit to the NRTSV experts

July 28-August 2 2015

IZSAM audition

7.

Conducting the final audit with a demonstration of acquired knowledge, skills, results in the project "Twining Laboratory" IZSAM visit to the NRTSV experts, report on corrective activity.

December 1-2 2015

IZSAM and USDA  audition

8.

Conducting the final international workshop on brucellosis. Reports on the results of the project – "Twining lab."

December 3-4 2015

Invited experts of veterinary organizations from Kazakhstan, Kyrgyzstan, Tajikistan, Uzbekistan, Experts and  auditors from IZSAM Institute, Italy and expert auditors from USDA

9.

Providing OIE with a report carried out within the framework of the project

December 2015

Experts auditors from IZSAM Institute, Italy; USDA expert auditors, RSE "NRTSV" Management

2. CAP-3 project.

In 2014-2015 the RSE ''NRTSV'' conducted an international project CAP-3 on "integration of archived samples to be used for supervision of the bluetongue, Akabane and Schmallenberg infections.

The Agreement was signed between the Delegation of "Technology Management Inc. Company'' (hereinafter - «TMC») and RSE "National Reference Center for Veterinary Medicine."

2.1. Project Overview

The Program on Biological Threat Reduction (PUBU) and US Defense Threat Reduction Agency (AGC MO US) in Kazakhstan, based on molecular methods to identify accompanying disease vectors have been introduced in Joint Biological Studies (BIS), including KZ-4, KZ 29, TAP-2, TAP-4, and surveillance within the framework of the program of detection of dangerous biological agents (OOBR).

AGC MO US is also funding the construction of the Central Reference Laboratory (CRL) in the Kazakh Scientific Center for Quarantine and Zoonotic Diseases (KSCQZD) in Almaty, Kazakhstan, the certified laboratories of 3rd biosafety level (BSL-3) and the Depositary for the protection of collections of highly dangerous pathogens (PLO).

CRL would be a major national diagnostic and reference agency, combined with modern (immunoanalysis and genetics) diagnostics, sequencing, and traditional microbiological methods. Since the activity of CRL Projects (CAP) to be completed on schedule before the commissioning of the CRL, CAP studies provide expertise to further identify and study gaps for a smoother transfer of CRL into operating condition.

CAP-3 particularlly includes an assessment of archived serum samples for screening using a newly developed diagnostic tests to monitor the relevant emerging infections that can affect Kazakhstan. The results of this research in future will help to develop and strengthen the institute procedures for disease surveillance, sample storage, and biosafety.

CAP-3 is a one-year study, which include a budget for materials and reagents. Research conducted by the RSE "NRTSV", Kazakhstani Institute and collaborators from the USA supported by AECOM. Project collaborators from the United States, which are already engaged in the ISF projects will connect participants from the Institute for the implementation of the required analysis and to strengthen surveillance systems for the identification of pathogens of diseases at an earlier stage and with a greater accuracy. In addition, the required baseline data will help to compare the available historical data in different institutions and future data to determine the true extent of the problems caused by pathogen data. Special attention will be payed to strengthen the supervisory framework required for rapid identification of any outbreak of archived samples.

2.2. Current progress

All laboratory tests were completed at the end of May 2015. In addition, July theses would be reported to CRWAD meeting and sent to the AGC MO USA; comments to AGC MO were included in the edited report. The project itself, in accordance with the schedule, was completed on October 2, 2015. According to this report, and the report on the results of research presented  on international conference CRWAD (Chicago, December 6-8).

3. Project TAP-7.

 In 2015 RSE NRTSV conducted an international project ''TAP-7'' on "Outreach work with the population to improve understanding of the ecology and epidemiology of African swine fever (ASF) in Kazakhstan, Georgia, Armenia and Ukraine: training and implementing methods for monitoring and prevention. This agreement has been concluded between the ''Technology Management Company Inc.'' (hereinafter  - ''TMC'') and RSE" National Reference center for veterinary Medicine. "

3.1. Project Overview

The development of an integrated and stable operating network of knowledge, expertise and "know-how" in the control and prevention of infections African swine fever (ASF) within the project in the framework of OOBR through joint efforts, which includes four countries (Armenia, Georgia , Kazakhstan, and Ukraine) in general plan of scientific work (Form A) on the basis of partnership.

There is a need for identification of gaps in knowledge, the legal framework and readiness for outbreaks . As this pathogen has a great potential for bioterrorism, and has great significance for human and animal health, and / or is deemed to cause significant instability in the economy, it is critical for countries endangered by ASF, regarding accurate and rapid detection and reporting to international health organizations, as well as providing response measures against the spread of the virus. This project will attempt to identify the key human resources and veterinary system in order to start implementing solutions to address identified gaps, thus increasing the opportunities to mitigate the risk of ASF.

3.2. Objective: Training and implementation of stable functioning of the education campaign to raise public awareness of the ASF, which aims to:

3.3. Project implementation:

- Regional joint working groups have been set up by the ASF, including representatives from Armenia, Georgia, Kazakhstan, and Ukraine and other officials from the International Veterinary and Food Bureau.

- Key groups to effectively implement the necessary methods to reduce and spread of ASF (veterinarians, farm workers, heads of Resource Management Services Wildlife and huntsmen) were identified.

- Through collaboration with the FAO and experts there have been created and distributed educational materials for key personnel in the areas and regions with risk of ASF infection, as well as seminars with the veterinary services of the IPI, employees of pig farms, the heads of service for wildlife management and huntsmen, as well as door-bypass (Figure 1).

Fig. 1 - Areas of ASF risk in Kazakhstan and the Russian Federation

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3.3.1. Conducting seminars and distribution of handouts in the Republic of Kazakhstan:

- Seminars were held in the Western Kazakhstan region in September 2015 in 7 areas bordering the Russian Federation and major pig farms.

Totally granted: 2800 leaflets and 51 posters.

- In Aktobe region during August 2015 in 5 districts bordering the Russian Federation and major pig farms.

Totally granted: 320 leaflets and 25 posters.

- In Kostanay region in 5 districts bordering the Russian Federation and major pig farms.

Totally granted: 1170 leaflets and 25 posters.

- In North-Kazakhstan region September 2015 8 areas bordering the Russian Federation and major pig farms.

Totally granted: 3,000 leaflets and 75 posters.

4. KZ-2 - "Ecology and epidemiology of brucellosis in South-Eastern Kazakhstan."

In this joint project included the following American institutions, Technology Management Company Inc. (hereinafter - «TMC»), from Kazakhstan "National Reference Center for Veterinary Medicine" KVKiN Ministry of Agriculture, State Enterprise - "Scientific and practical center of sanitary-epidemiological examination and monitoring'', the State Enterprise - "Kazakh Scientific center for quarantine and zoological Diseases after M. Aykimbaeva", Ministry of Health of the Republic of kazakhstan.

Long-term project has been divided into two phases, Phase One of the project was carried out in the period from 2005 to 2009, Phase Two started in 2012 and completed in April 2014.

The project was financed by the American side, including salaries for specialists, performing direct work, the supply of reagents and materials required for the project, flights to international conferences, seminars and trainings.

During Phase Two 21 specialists from RSE "NRTSV" attended the project.

The collaborators of the project were specialists of the Research Institute. US Walter Reed Army troops; Texas Agricultural and Mechanical University (TAMU), USA; Louisiana State University, Department of Veterinary Science, the Center for cooperation and use of remote radar and Global Information Health System of the World Health Organization, the United States Department of Geography and Anthropology, Louisiana State University, USA.

4.1. Main objectives of the Project were: effective training and implementation of standard epidemiological methods, including a reporting system through ''EISNZ'', which will serve as the further integrator of the activities of the BIS and TADR; introducing additional scientific methods, such as analysis optimization qPCRi that provide the sequence of previous activities (eg, MLVA-8); assist institutions in developing procedures to improve the system responding to outbreaks of disease both for humans and animals, and strengthen cooperation in responding to such outbreaks; accelerate the consolidation of TELO strains and preparation activities of the CRL on the basis of the State Enterprise "KSCQZD"; improving skills and the key scientific capacity in the relevant institutions for current and future projects of the ISF in order to ensure long-term sustainable development; development and implementation of a unified system for disease surveillance that can be fully used by institutions on their own purposes, providing results meeting the aforementioned goals, including peer-reviewed articles publication, cooperation with the international scientific community and other work aimed at obtaining additional funding from the outside.

In 2014, from April 15 to 26 the continuous scientific work to analyze VNTR / MLVA Brucella species through a system of genetic analysis Beckman CEQ was performed. On the basis of NPTSSEEM together with collaborators, doctors Nikolic and Huang were conducted and completed basic research on sequencing of strains within the 1st phase and within the 2nd phase of the project.

Carrying out work for implementation of the Phase Two MLVA 15 NRTSV participated in conjunction with NPTSSEEM experts. The multiplex reaction carried out on five pairs of primers for pair 3 and 7 primer pairs. The medical and veterinary isolates of Brucella were studied and analyzed together with US collaborators. Total studied  - multiplex reaction of 15 primer pairs, 67 isolates of health within the Phase Two, with the remaining 19 strains of the Phase One, as well as veterinary isolates.

NPTSSEEM Experts in collaboration with Dr. Jason Blackburn continued to work on GIS from March 17 to 20 2014., Introducing geographic data on the map.

At the same time NRTSV branch based in Astana performed phage typing training.

5. TAP-4 - "Application to the approach of differentiation of infected and vaccinated animals (DIVA) for the purpose of surveillance of foot and mouth disease in Kazakhstan."

The joint project included the following institutions from the American side, - Technology Management Company Inc., from the Kazakhstani side RSE "National Reference Center for Veterinary Medicine" KVKiN the Ministry of Agriculture.

The program on reduction of biological threat AGC (PUBU) Detection System hazardous bioreagents and response (OOBR) also supported the diagnostic protocols, which include the identification of FMD by qPTsR and supervision over the use of serum by ELISA.

In the project, to review important aspects of surveillance for FMD disease, including the identification of non-structural proteins (NSP) in animal serum as the infection index and comparison of genomic sequences during outbreaks in Kazakhstan, provided the use of an DIVA approach (differentiation of infected and vaccinated animals).

In survey the data and samples used from recent outbreaks of foot and mouth disease sites, including serum samples obtained from noted zones free from FMD endemic zones and epidemics in the Republic of Kazakhstan.

The period of implementation of the project took place since January 1, 2011 until June 2013.

These studies are of great importance for the detection and analysis of the epizootic situation in the country as a whole and for further implementation of preventive measures against FMD required to monitor new outbreaks. To conduct a comparative analysis of the probability of detection of antigens in vaccinated and unvaccinated animals in the framework of the project TAP -4.

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